PMID:7523833
| Citation |
He, L, Söderbom, F, Wagner, EG, Binnie, U, Binns, N and Masters, M (1993) PcnB is required for the rapid degradation of RNAI, the antisense RNA that controls the copy number of ColE1-related plasmids. Mol. Microbiol. 9:1131-42 |
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| Abstract |
The replication of ColE1-related plasmids is controlled by an unstable antisense RNA, RNAI, which can interfere with the successful processing of the RNAII primer of replication. We show here that a host protein, PcnB, supports replication by promoting the decay of RNAI. In bacterial strains deleted for PcnB a stable, active form of RNAI, RNAI*, which appears to be identical to the product of 5'-end processing by RNAase E, accumulates. This leads to a reduction in plasmid copy number. We show, using a GST-PcnB fusion protein, that PcnB does not interfere with RNAI/RNAII binding in vitro. The fusion protein, like PcnB, has polyadenylating activity and is able to polyadenylate RNAI (and also another antisense RNA, CopA) in vitro. |
| Links | |
| Keywords |
Ampicillin Resistance/genetics; Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; Bacterial Proteins/metabolism; Base Sequence; Blotting, Northern; Colicins/antagonists & inhibitors; DNA Primers; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Bacterial; Kinetics; Molecular Sequence Data; Plasmids; Polymerase Chain Reaction; Polynucleotide Adenylyltransferase/metabolism; RNA, Antisense/genetics; RNA, Antisense/isolation & purification; RNA, Antisense/metabolism; RNA, Bacterial/genetics; RNA, Bacterial/metabolism; Recombinant Fusion Proteins/biosynthesis; Recombinant Fusion Proteins/metabolism; Transcription, Genetic |
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