PMID:7493980
| Citation |
Severinov, K, Mustaev, A, Severinova, E, Kozlov, M, Darst, SA and Goldfarb, A (1995) The beta subunit Rif-cluster I is only angstroms away from the active center of Escherichia coli RNA polymerase. J. Biol. Chem. 270:29428-32 |
|---|---|
| Abstract |
Ribonucleotide analogs bound in the initiating site of Escherichia coli RNA polymerase-promoter complex were cross-linked to the beta subunit. Using limited proteolysis and chemical degradation, the cross-link was mapped to a segment of beta between amino acids Val516 and Arg540. This region (Rif-cluster I) is known to harbor many rifampicin-resistant (RifR) mutations. The results demonstrate that Rif-culster I is part of the "5'-face" of the active center and provide structural basis for the long-known effects of RifR mutations on transcription initiation, elongation, and termination. |
| Links | |
| Keywords |
Binding Sites; DNA-Directed RNA Polymerases/chemistry; Drug Resistance, Microbial/genetics; Escherichia coli/enzymology; Peptide Mapping; Rifampin/pharmacology |
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