PMID:7489493
| Citation |
Santer, UV, Cekleniak, J, Kansil, S, Santer, M, O'Connor, M and Dahlberg, AE (1995) A mutation at the universally conserved position 529 in Escherichia coli 16S rRNA creates a functional but highly error prone ribosome. RNA 1:89-94 |
|---|---|
| Abstract |
A base substitution of G to U was constructed at position 529 in Escherichia coli 16S rRNA. The U529 mutant ribosomes were functional and present on polysomes but were highly error prone and caused a progressive loss of cell viability. They displayed elevated levels of readthrough of stop codons and frameshifting, and an increase in thermal sensitivity of beta-galactosidase, suggestive of missense errors. These results demonstrate that the university conserved G529 is involved in tRNA selection at the A site during protein synthesis. |
| Links | |
| Keywords |
Base Sequence; Conserved Sequence; Escherichia coli/genetics; Escherichia coli/growth & development; Hot Temperature; Molecular Sequence Data; Mutagenesis, Site-Directed; Mutation; Nucleic Acid Conformation; Polyribosomes/metabolism; Protein Biosynthesis; Protein Denaturation; RNA; RNA, Messenger/metabolism; RNA, Ribosomal, 16S/genetics; Ribosomes/metabolism; beta-Galactosidase/biosynthesis |
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