PMID:7050087

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Citation

Stewart, V (1982) Requirement of Fnr and NarL functions for nitrate reductase expression in Escherichia coli K-12. J. Bacteriol. 151:1320-5

Abstract

I used a chlC-lac operon fusion to study regulatory mutations which affect nitrate reductase expression in Escherichia coli. A NarL- mutant apparently lacks a nitrate-specific positive regulatory component. Furthermore, an fnr (nirR) mutation prevented enzyme induction under any conditions. These data are consistent with a two-step, positive control model for nitrate reductase regulation.

Links

PubMed PMC220410

Keywords

DNA, Recombinant; Escherichia coli/enzymology; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Genes, Regulator; Mutation; Nitrate Reductases/biosynthesis; Nitrate Reductases/genetics; Operon; Oxygen/pharmacology; Transduction, Genetic

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