PMID:7046803
| Citation |
Piffeteau, A, Zamboni, M and Gaudry, M (1982) Biotin transport by a biotin-deficient strain of Escherichia coli. Biochim. Biophys. Acta 688:29-36 |
|---|---|
| Abstract |
Biotin uptake has been investigated using an Escherichia coli biotin requiring auxotroph grown under biotin-deficient conditions. This strain accumulated biotin in the free and bound form. In agreement with a previous report by O. Prakash and M.A. Eisenberg (J. Bacteriol. 120 (1974) 785-791), the biotin entry proved to be an active process which depended on an energy source and was inhibited in the presence of uncouplers. The kinetic parameters have been determined (KM = 0.05 microM, Vmax = 7 pmol/min per mg dry weight). The pool of free biotin could be readily exchanged with external biotin and decreased to a very low level in the absence of an energy source. The use of several biotin analogues revealed that this transport system was quite specific for biotin: slight modifications, for instance in the valeric chain, lowered drastically the affinity for the carrier. |
| Links | |
| Keywords |
Biological Transport, Active; Biotin/analogs & derivatives; Biotin/deficiency; Biotin/metabolism; Cytoplasm/metabolism; Escherichia coli/metabolism; Kinetics; Structure-Activity Relationship |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.