PMID:7037743
| Citation |
Cronan, JE Jr, Littel, KJ and Jackowski, S (1982) Genetic and biochemical analyses of pantothenate biosynthesis in Escherichia coli and Salmonella typhimurium. J. Bacteriol. 149:916-22 |
|---|---|
| Abstract |
Pantothenate (pan) auxotrophs of Escherichia coli K-12 and Salmonella typhimurium LT2 were characterized by enzymatic and genetic analyses. The panB mutants of both organisms and the pan-6 ("panA") mutant of S. typhimurium are deficient in ketopantoate hydroxymethyltransferase, whereas the panC mutants lack pantothenate synthetase. panD mutants of E. coli K-12 were previously shown to be deficient in aspartate 1-decarboxylase. All mutants showed only a single enzyme defect. The finding that the pan-6 mutant was deficient in ketopantoate hydroxymethyltransferase indicates that the genetic lesion is a panB allele. The pan-6 mutant therefore is deficient in the utilization of alpha-ketoisovalerate rather than the synthesis of alpha-ketoisovalerate, as originally proposed. The order of the pan genes of E. coli K-12 was determined by phage P1-mediated three-factor crosses. The clockwise order was found to be aceF panB panD panC tonA on the genetic map of E. coli K-12. The three-factor crosses were greatly facilitated by use of a closely linked Tn10 transposon as the outside marker. We also found that supplementation of E. coli K-12 auxotrophs with a high concentration of pantothenate or beta-alanine increased the intracellular coenzyme A level two- to threefold above the normal level. Supplementation with pantoate or ketopantoate resulted in smaller increases. |
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| Keywords |
Carboxy-Lyases/genetics; Chromosome Mapping; Chromosomes, Bacterial; Coenzyme A/metabolism; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Hydroxymethyl and Formyl Transferases; Pantothenic Acid/biosynthesis; Pantothenic Acid/genetics; Peptide Synthases/genetics; Salmonella typhimurium/genetics; Salmonella typhimurium/metabolism; Transferases |
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