PMID:7034716
| Citation |
Hayzer, DJ and Leisinger, T (1981) Proline biosynthesis in Escherichia coli. Stoichiometry and end-product identification of the reaction catalysed by glutamate semialdehyde dehydrogenase. Biochem. J. 197:269-74 |
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| Abstract |
The stoichiometry of the oxidative phosphorylation of glutamic acid 5-semialdehyde by gamma-glutamyl phosphate reductase (glutamate semialdehyde dehydrogenase) has been established. Equimolar amounts of NADP+ and L-glutamic acid 5-semialdehyde are consumed and equimolar amounts of 5-oxiopyrroilidine-2-carboxylic acid and NADPH are formed. The end-product of the reaction is demonstrated to be 5-oxopyrrolidine-2-carboxylic acid, probably arising from the true end-product gamma-glutamyl phosphate. |
| Links | |
| Keywords |
Aldehyde Oxidoreductases/metabolism; Chemical Phenomena; Chemistry; Chromatography, Thin Layer; Escherichia coli/metabolism; Glutamate-5-Semialdehyde Dehydrogenase; NADP/metabolism; Proline/biosynthesis; Pyrrolidonecarboxylic Acid/metabolism |
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