PMID:7028715

Citation	Kolodrubetz, D and Schleif, R (1981) L-arabinose transport systems in Escherichia coli K-12. J. Bacteriol. 148:472-9
Abstract	Mutations in the arabinose transport operons of Escherichia coli K-12 were isolated with the Mu lac phage by screening for cells in which beta-galactosidase is induced in the presence of L-arabinose. Standard genetic techniques were then used to isolate numerous mutations in either of the two transport systems. Complementation tests revealed only one gene, araE, in the low-affinity arabinose uptake system. P1 transduction placed araE between lysA (60.9 min) and thyA (60.5 min) and closer to lysA. The operon of the high-affinity transport system was found to contain two genes: araF, which codes for the arabinose-binding protein, and a new gene, araG. The newly identified gene, araG, was shown by two-dimensional gel electrophoresis to encode a protein which is located in the membrane. Only defects in araG could abolish uptake by the high-affinity system under the conditions we used.
Links	PubMed PMC216229
Keywords	Arabinose/genetics; Arabinose/metabolism; Bacterial Proteins/genetics; Biological Transport; Carrier Proteins/genetics; Chromosome Mapping; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Genes, Bacterial; Genetic Complementation Test; Mutation
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