PMID:7017348
| Citation |
Bendiak, DS and Friesen, JD (1981) Organization of genes in the four minute region of the Escherichia coli chromosome: evidence that rpsB and tsf are co-transcribed. Mol. Gen. Genet. 181:356-62 |
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| Abstract |
Restriction endonuclease-generated DNA fragments of lambda polC-9 (Friesen et al. 1976) have been cloned on plasmid vehicles. Expression of genes carried by these plasmids was determined either by genetic complementation of the appropriate mutants, or in ultraviolet-irradiated cells. On the basis of these experiments we have inferred the following gene order in the four minute region of the Escherichia coli chromosome: tonA-dapD4-dapD2-rpsB-tsf-22 kilodalton protein - fir 27,000-firA-dnaE. We suggest that rpsB and tsf are in one transcriptional unit, with rpsB being promoter-proximal. We also suggest the possible position of the promoter for dnaE. |
| Links | |
| Keywords |
Chromosomes, Bacterial/ultrastructure; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Gene Expression Regulation; Genes; Genetic Linkage; Operon; Peptide Elongation Factors/genetics; Plasmids; Ribosomal Proteins/genetics; Transcription, Genetic |
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