PMID:7007049

Citation	Thomas, G and Favre, A (1980) 4-Thiouridine triggers both growth delay induced by near-ultraviolet light and photoprotection. Eur. J. Biochem. 113:67-74
Abstract	4-Thiouridine, a rare nucleoside present in Escherichia coli tRNAs, has been recently proposed to be the major chromophore leading to near-ultraviolet (315-400-nm)-induced growth delay. Here this is established by the isolation of mutants exhibiting a reduced growth delay. The selection procedure involves several successive cycles of 365-nm illumination of the cells in the stationary phase, followed by growth for two or three generations. After the eighth cycle, the level of 4-thiouridine in the culture decreases to 20% of the original level and all individual clones tested show a 4-thiouridine deficiency. One mutant exhibiting a complete lack of 4-thiouridine in its tRNAs has been characterized. In the dark the growth characteristics of the mutant and of the parental strain are indistinguishable. In contrast after near-ultraviolet illumination the nuv mutation abolishes the growth delay and considerably reduces the photoprotection efficiency.
Links	PubMed
Keywords	Escherichia coli/genetics; Escherichia coli/growth & development; Escherichia coli/radiation effects; Mutation; Photolysis; RNA, Transfer/analysis; Thionucleotides/analysis; Thiouridine/metabolism; Ultraviolet Rays; Uridine/analysis
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