PMID:6998952
| Citation |
Carl, PL, Bloom, L and Crouch, RJ (1980) Isolation and mapping of a mutation in Escherichia coli with altered levels of ribonuclease H. J. Bacteriol. 144:28-35 |
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| Abstract |
A mutant of Escherichia coli with altered levels of ribonuclease (RNase) H was isolated after mutagenesis with ethyl methane sulfonate. A procedure for assaying RNase H in partially purified extracts was used to screen approximately 1,500 colonies for variations in RNase H activity. Confirmation of a lower level of RNase H in the mutant was accomplished by analysis of RNase H in sodium dodecyl sulfate-polyacrylamide gels. By Hfr, F', and P1 transduction mapping, the genetic locus responsible for the lower levels of RNase H was located at 5.1 min on the E. coli chromosome. This mutation (rnh) represents a new locus on the E. coli chromosome. The only phenotypic characteristic of this mutation which has been observed to date is the lower level of RNase H (30% of parental values). |
| Links | |
| Keywords |
Chromosome Mapping; Chromosomes, Bacterial; DNA Replication; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Mutation; Plasmids; Ribonucleases/genetics |
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