PMID:6989798
| Citation |
Roehl, RA and Vinopal, RT (1980) Genetic locus, distant from ptsM, affecting enzyme IIA/IIB function in Escherichia coli K-12. J. Bacteriol. 142:120-30 |
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| Abstract |
Most strains of Escherichia coli K-12 are unable to use the enzyme IIA/IIB (enzyme IIMan) complex of the phosphoenolpyruvate:sugar phosphotransferase system (PTS) in anaerobic growth and therefore cannot utilize glucosamine anaerobically. Introduction into these strains of a ptsG mutation, which eliminates activity of the enzyme IIIGlc/IIB' complex of the PTS, resulted in inability to grow anaerobically on glucose and mannose. Derivative strains able to grow anaerobically on glucosamine had mutations at a locus close to man, the gene coding for phosphomannose isomerase, and had higher enzyme IIA/IIB activities during anaerobic growth than did the parental strain. These results establish a locus affecting function of enzyme IIA/IIB that maps distant from ptsM, the probable structural gene for enzyme IIB. |
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| Keywords |
Anaerobiosis; Biological Transport; Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Glucosamine/metabolism; Glucose/metabolism; Mannose/metabolism; Mutation; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism |
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