PMID:6787390

Citation	Levine, RA and Taylor, MW (1981) Selection for purine regulatory mutants in an E. coli hypoxanthine phosphoribosyl transferase-guanine phosphoribosyl transferase double mutant. Mol. Gen. Genet. 181:313-8
Abstract	We have studied the relationship betwen purine salvage enzymes, 6-mercaptopurine resistance, and the purR phenotype in E. coli. Mutants resistant to 6-mercaptopurine were found to have defects in HPRT, the purR repressor, or in both. Analysis of these mutants led to the isolation of a hypoxanthine phosphoribosyl transferase-guanine phosphoribosyl transferase double mutant (hpt- gpt-) that is extremely sensitive to adenine. Two classes of adenine resistant mutants were isolated from this strain. The first class was deficient in APRT (apt-) while the second class represented purine regulatory mutants (purR-). There is thus selection for the purR phenotype in a hpt- gpt- background.
Links	PubMed
Keywords	Adenine/pharmacology; Adenine Phosphoribosyltransferase/genetics; Escherichia coli/genetics; Genes, Regulator; Hypoxanthine Phosphoribosyltransferase/genetics; Mutation; Pentosyltransferases/genetics; Purines/metabolism; Selection, Genetic
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