PMID:6761148
| Citation |
Barker, DG, Bruton, CJ and Winter, G (1982) The tyrosyl-tRNA synthetase from Escherichia coli. Complete nucleotide sequence of the structural gene. FEBS Lett. 150:419-23 |
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| Abstract |
The structural component of the tyrS gene of Escherichia coli, comprising 1269 base pairs, has been fully sequenced by the combined M13/dideoxychain termination approach. The gene has a codon usage pattern which is typical of highly expressed proteins and similar to other Escherichia coli aminoacyl-tRNA synthetase genes. Peptide purification and sequencing has been used to locate the N-terminus and to provide confirmation of 95% of the translated protein sequence. This latter yields on Mr of 47,403 for the Escherichia coli tyrosyl-tRNA synthetase, and reveals considerable homology with the primary structure of the analogous enzyme isolated from Bacillus staerothermophilus. |
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| Keywords |
Amino Acid Sequence; Amino Acyl-tRNA Synthetases/genetics; Base Sequence; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Tyrosine-tRNA Ligase/genetics |
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