PMID:6759505
| Citation |
Mizugaki, M, Kimura, C, Nishimaki, T, Yamamoto, H, Sagi, M, Nishimura, S and Yamanaka, H (1982) Studies on the metabolism of unsaturated fatty acids. X. Purification and some properties of 2,4-dienoyl-CoA reductase from Escherichia coli. J. Biochem. 92:1671-4 |
|---|---|
| Abstract |
2,4-Dienoyl-CoA reductase has been separated from Escherichia coli grown in the presence of linoleic acid and purified to homogeneity. The enzyme has a molecular weight close to 50,000 as determined by gel filtration on Sephacryl S-200 Super-fine. The reductase was rather stable in a buffer containing citric acid and kept its full activity on heating at 55 degrees C for 10 min in the pH range of 5.5 to 6.5, but was completely inactivated on heating at 58 degrees C for 10 min. Phosphocellulose column chromatography revealed that the reductase was not involved in the multi-enzyme complex (molecular weight of 260,000) of fatty acid oxidation. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Fatty Acid Desaturases/isolation & purification; Fatty Acid Desaturases/metabolism; Fatty Acids, Unsaturated/metabolism; Hot Temperature; Kinetics; Molecular Weight |
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