PMID:6749299
| Citation |
Yen, TS and Webster, RE (1982) Translational control of bacteriophage f1 gene II and gene X proteins by gene V protein. Cell 29:337-45 |
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| Abstract |
The gene II region of bacteriophage f1 DNA codes for two proteins, the 46 kd gene II protein and the 13 kd gene X protein, which results from an in-phase start at codon 300 of gene II. Using antigene II protein IgG, we show that the intracellular concentration of both proteins is controlled by the phage gene V protein. In wild-type f1-infected cells, the amount of gene II protein reaches a plateau of about 1500 molecules per cell at 20 min after infection, as measured by blot immunoassay. Similarly, the amount of gene X protein reaches a peak of about 500 molecules per cell around 10 min after infection. In contrast, when the gene V protein is inactive, both gene II and gene X proteins continue to accumulate at a high rate for at least 40 min after infection. This difference is caused by decreased synthesis of gene II and gene X proteins in the presence of gene V protein, which represses the translation of these two proteins. |
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| Keywords |
Antibodies, Viral; Coliphages/genetics; Escherichia coli/metabolism; Gene Expression Regulation; Mutation; Protein Biosynthesis; RNA, Messenger/genetics; RNA, Messenger/metabolism; Viral Proteins/biosynthesis; Viral Proteins/immunology; Viral Proteins/physiology |
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