PMID:6460914
| Citation |
Bienkowska-Szewczyk, K, Lipinska, B and Taylor, A (1981) The R gene product of bacteriophage lambda is the murein transglycosylase. Mol. Gen. Genet. 184:111-4 |
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| Abstract |
The radioactively labeled proteins synthesised in Escherichia coli minicells infected by bacteriophage lambda R and lambda R+ were compared by polyacrylamide gel electrophoresis. lambda R mutants, which have lost the ability to lyse host cells, lack a polypeptide of molecular weight 17.5 KD corresponding to the molecular weight of murein transglycosylase - a bacteriolytic enzyme from lambda lysates which we have described previously. It has been shown by direct comparison using radio-labeled enzyme that transglycosylase comigrates with the R gene product. The enzyme was undetectable in induced cultures of E. coli W3350 su degrees (lambda cI857 Ram 5) and C600 (lambda cI857 acR301), while it was present in a lambda Rz mutant lysate. We conclude that the transglycosylase is the R gene product. |
| Links | |
| Keywords |
Bacteriophage lambda/genetics; Electrophoresis, Polyacrylamide Gel; Escherichia coli/genetics; Genes, Bacterial; Genes, Viral; Glycosyltransferases; Peptidoglycan; Protein Biosynthesis; Transferases/genetics |
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