PMID:6450748
| Citation |
Botta, GA and Park, JT (1981) Evidence for involvement of penicillin-binding protein 3 in murein synthesis during septation but not during cell elongation. J. Bacteriol. 145:333-40 |
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| Abstract |
Furazlocillin (1 microgram/ml) and piperacillin (5 microgram/ml) bound specifically to penicillin-binding protein 3 (PBP-3) and not to the other major PBPs in intact Escherichia coli cells. The effect of this specific binding to PBP-3 on murein synthesis of elongating and synchronously septating cells was investigated in two thermosensitive division mutants, E. coli BUG6 and E. coli JE10730, the latter possessing a thermolabile PBP-3. Synchronous cell division was induced by shifting the cultures from the nonpermissive temperature (42 degrees C) to 30 degrees C. Both [14C]diaminopimelic acid incorporation into murein of intact cells and [14C]N-acetylglucosamine incorporation into murein of cells permeabilized with ether was inhibited by an average of 42% in septating cells. In filaments growing at the nonpermissive temperature, we detected no inhibition and, frequently, a 10 to 15% stimulation of murein synthesis. The two drugs, at concentrations used in the above experiments, bound exclusively to PBP-3 both in elongating and septating intact cells and in ether-treated cells. These results support the hypothesis that PBP-3 activity is exclusively required for septal murein synthesis. |
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| Keywords |
Azlocillin/analogs & derivatives; Bacterial Proteins; Carrier Proteins/physiology; Cell Division; Chloramphenicol/pharmacology; Escherichia coli/growth & development; Escherichia coli/metabolism; Escherichia coli Proteins; Hexosyltransferases; Imidazolidines; Muramoylpentapeptide Carboxypeptidase; Penicillin-Binding Proteins; Penicillins/metabolism; Penicillins/pharmacology; Penicillins/physiology; Peptidoglycan/biosynthesis; Peptidoglycan Glycosyltransferase; Peptidyl Transferases; Piperacillin |
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