PMID:6449635
| Citation |
Ardeshir, F and Ames, GF (1980) Cloning of the histidine transport genes from Salmonella typhimurium and characterization of an analogous transport system in Escherichia coli. J. Supramol. Struct. 13:117-30 |
|---|---|
| Abstract |
The genes for the well-characterized high-affinity histidine transport system of S typhimurium have been cloned in lambda gt4. Genetic and physiological analyses of the analogous transport system of E coli were undertaken in order that available lambda vectors, recombinant DNA techniques, and a genetic selection for transport function might be used to isolate the Salmonella genes. The presence of the transport genes on a 12.4 Kb cloned DNA fragment has been confirmed 1) genetically, by complementation studies; 2) physiologically, by the rates of histidine uptake by bacteria containing this DNA; and 3) by demonstrating that the cloned DNA codes for the previously identified transport proteins J and P. The isolated fragment carries the entire transport operon, the argT gene and the ubiX locus, but neither the purF gene nor the ack/pta loci. |
| Links |
PubMed Online version:10.1002/jss.400130111 |
| Keywords |
Bacteriophage lambda/metabolism; Biological Transport; Carrier Proteins/metabolism; Cloning, Molecular; DNA, Recombinant/metabolism; Escherichia coli/metabolism; Genes; Genetic Complementation Test; Genotype; Histidine/metabolism; Operon; Periplasmic Binding Proteins; Salmonella typhimurium/genetics; Salmonella typhimurium/metabolism; Species Specificity |
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