PMID:6444453
| Citation |
Büchel, DE, Gronenborn, B and Müller-Hill, B (1980) Sequence of the lactose permease gene. Nature 283:541-5 |
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| Abstract |
The nucleotide sequence of the lacY gene coding for lactose permease (M protein) in Escherichia coli has been determined. The sequence includes the intergenic regions between the lacZ (beta-galactosidase) and lacY genes as well as the region between the lacY and lacA (transacetylase) genes. Lactose permease is predicted to consist of 417 residues (71% nonpolar), resulting in a protein with a molecular weight of 46,504. The reading frame was confirmed by the sequence of a nonsense mutation changing codon 33 from UGG to UAG. |
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| Keywords |
Amino Acid Sequence; Bacteriophage lambda/genetics; Base Sequence; Binding Sites; DNA, Bacterial/genetics; DNA, Recombinant; Escherichia coli/genetics; Genes; Lac Operon; Membrane Transport Proteins/genetics; Ribosomes/metabolism |
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