PMID:6427754
| Citation |
Elseviers, D, Petrullo, LA and Gallagher, PJ (1984) Novel E. coli mutants deficient in biosynthesis of 5-methylaminomethyl-2-thiouridine. Nucleic Acids Res. 12:3521-34 |
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| Abstract |
Novel E. coli mutants deficient in biosynthesis of 5- methylaminomethyl -2-thiouridine were isolated based on a phenotype of reduced readthrough at UAG codons. They define 2 new loci trmE and trmF , near 83' on the E. coli map. These mutants are different from strains carrying trmC mutations, which are known to confer a methylation deficiency in biosynthesis of 5- methylaminomethyl -2-thiouridine. tRNA from mutants carrying trmE or trmF mutations was shown to carry 2-thiouridine instead of 5- methylaminomethyl -2-thiouridine. This deficiency affects the triplet binding properties of the mutant tRNA. Our results suggest that the 5- methylaminomethyl group stabilizes the basepairing of this modified nucleotide with G, most likely through direct interaction with the ribosomal binding site(s). |
| Links | |
| Keywords |
Base Sequence; Codon; Escherichia coli/genetics; Genotype; Mutation; Phenotype; RNA, Transfer/genetics; Species Specificity; Thiouridine/analogs & derivatives; Thiouridine/metabolism; beta-Galactosidase/genetics |
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