PMID:6425270
| Citation |
Rosenberg, H, Pearce, SM, Hardy, CM and Jacomb, PA (1984) Rapid turnover of mannitol-1-phosphate in Escherichia coli. J. Bacteriol. 158:63-8 |
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| Abstract |
The phosphate moiety of D-mannitol-1-phosphate in Escherichia coli is subject to rapid turnover and is in close equilibrium with Pi and the phosphorus of fructose-1,6-bisphosphate. These three compounds account for the bulk of 32P label found in cells after several minutes of uptake of 32Pi and mannitol-1-phosphate represents some 30% of this label. Mannitol-1-phosphate occurs in E. coli grown on a variety of carbon sources, in the absence of D-mannitol, and is synthesized de novo even in mutants lacking mannitol-1-phosphate dehydrogenase. The mannitol moiety of mannitol-1-phosphate was not affected during the total chase of the P moiety, which exchanged with a half-life of about 30 s. These findings suggest that the rapid equilibration of the phosphorus is a function of an enzyme, possibly a component of the phosphotransferase system, capable of forming a complex that allows the exchange of the phosphate without the equilibration of the mannitol moiety with free mannitol. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/metabolism; Escherichia coli Proteins; Fructosediphosphates/metabolism; Kinetics; Mannitol/metabolism; Mannitol Phosphates/metabolism; Monosaccharide Transport Proteins; Mutation; Phosphates/metabolism; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Phosphoenolpyruvate Sugar Phosphotransferase System/metabolism; Sugar Alcohol Dehydrogenases/genetics; Sugar Alcohol Dehydrogenases/metabolism; Sugar Phosphates/metabolism |
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