PMID:6425119

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Citation

Band, L, Shimotsu, H and Henner, DJ (1984) Nucleotide sequence of the Bacillus subtilis trpE and trpD genes. Gene 27:55-65

Abstract

Several overlapping portions of the tryptophan (trp) operon of Bacillus subtilis have been cloned into plasmid pBR322. The nucleotide sequence of the region comprising the trpE and trpD genes and a portion of the trpC gene has been determined. When the deduced amino acid sequences of these genes are compared with their counterparts in Escherichia coli, several regions of striking homology are seen. The probable initiation codons for the trpE, D and C genes are each preceded by a recognizable Shine-Dalgarno sequence. The coding sequences for the trpE and trpD genes and for the trpD and trpC genes overlap slightly, leaving no intercistronic regions between the genes.

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Keywords

Amino Acid Sequence; Anthranilate Phosphoribosyltransferase/genetics; Anthranilate Synthase/genetics; Bacillus subtilis/genetics; Bacterial Proteins/genetics; Base Sequence; Cloning, Molecular; Escherichia coli/genetics; Gene Expression Regulation; Genes, Bacterial; Operon; Pentosyltransferases/genetics; Species Specificity

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