PMID:6397464
| Citation |
Homma, H, Kobayashi, T, Chiba, N, Karasawa, K, Mizushima, H, Kudo, I, Inoue, K, Ikeda, H, Sekiguchi, M and Nojima, S (1984) The DNA sequence encoding pldA gene, the structural gene for detergent-resistant phospholipase A of E. coli. J. Biochem. 96:1655-64 |
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| Abstract |
The nucleotide sequence of the pldA gene of Escherichia coli K-12, which codes for detergent-resistant phospholipase A (DR-phospholipase A), located in the outer membrane, was determined and the amino acid sequence of DR-phospholipase A was deduced. DR-phospholipase A contains 269 amino acids, resulting in a protein with a molecular weight of 30,809. It does not contain any cysteine residues and seems to be synthesized first as a precursor with a typical signal peptide composed of 20 amino acids. The NH2-terminus of the mature protein is glutamine, a polar amino acid, while other outer membrane proteins so far determined have a nonpolar amino acid there. The hydropathy profile of the deduced amino acid sequence of DR-phospholipase A was studied. Most of the region was rather hydrophilic and there were no stretches of hydrophobic amino acids. Computer analysis showed that there are no homologies between DR-phospholipase A and other extracellular phospholipases whose amino acid sequences are known. The candidates for the promoter region of the pldA gene, the 5'-flanking region, have a significantly high AT content, while the AT content of the coding region is about the same as the average AT content of the E. coli chromosome. A typical rho-independent transcription termination site is also present at the 3'-flanking region. This is the first example of the primary structure of a membrane-bound phospholipase. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Codon; DNA, Bacterial/isolation & purification; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Peptides/analysis; Peptides/genetics; Phospholipases/biosynthesis; Phospholipases A/analysis; Phospholipases A/biosynthesis; Plasmids; Protein Sorting Signals |
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