PMID:6395895
| Citation |
Hudson, GS, Wong, V and Davidson, BE (1984) Chorismate mutase/prephenate dehydrogenase from Escherichia coli K12: purification, characterization, and identification of a reactive cysteine. Biochemistry 23:6240-9 |
|---|---|
| Abstract |
The bifunctional enzyme involved in tyrosine biosynthesis, chorismate mutase/prephenate dehydrogenase, has been isolated from extracts of a regulatory mutant of Escherichia coli K12. The pure enzyme is a homodimer of total molecular weight 78 000 and displays Michaelis-Menten kinetics for both activities. Fingerprinting and amino acid sequencing of tryptic and thermolytic peptides of the S-[14C]carboxymethylated enzyme allowed the identification of three unique cysteine-containing sequences per subunit. Chemical modification of the native enzyme with 5,5'-dithiobis(2-nitrobenzoate) or iodoacetamide showed that one sulfhydryl group per subunit was particularly reactive, and the integrity of this group was essential for both enzymic activities. This work supports previous proposals for a close spatial relationship between the active sites. |
| Links | |
| Keywords |
Amino Acid Sequence; Centrifugation; Chemical Phenomena; Chemistry; Chorismate Mutase/isolation & purification; Chorismate Mutase/metabolism; Chromatography, Affinity; Cysteine; Dithionitrobenzoic Acid; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Iodoacetamide; Isomerases/metabolism; Kinetics; Molecular Weight; Oxidoreductases/metabolism; Peptide Fragments/isolation & purification; Prephenate Dehydrogenase/isolation & purification; Prephenate Dehydrogenase/metabolism; Thermolysin; Trypsin |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.
