PMID:6394957
| Citation |
Boeke, JD, LaCroute, F and Fink, GR (1984) A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol. Gen. Genet. 197:345-6 |
|---|---|
| Abstract |
Mutations at the URA3 locus of Saccharomyces cerevisiae can be obtained by a positive selection. Wild-type strains of yeast (or ura3 mutant strains containing a plasmid-borne URA3+ gene) are unable to grow on medium containing the pyrimidine analog 5-fluoro-orotic acid, whereas ura3- mutants grow normally. This selection, based on the loss of orotidine-5'-phosphate decarboxylase activity seems applicable to a variety of eucaryotic and procaryotic cells. |
| Links | |
| Keywords |
Carboxy-Lyases/genetics; Drug Resistance, Microbial; Fungal Proteins/genetics; Microbiological Techniques; Orotic Acid/analogs & derivatives; Orotic Acid/pharmacology; Orotidine-5'-Phosphate Decarboxylase/genetics; Saccharomyces cerevisiae/drug effects; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae/genetics |
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