PMID:6387713
| Citation |
Baker, TA, Grossman, AD and Gross, CA (1984) A gene regulating the heat shock response in Escherichia coli also affects proteolysis. Proc. Natl. Acad. Sci. U.S.A. 81:6779-83 |
|---|---|
| Abstract |
The htpR locus in Escherichia coli encodes a regulator of the heat shock response. Cells containing the htpR165 mutation are defective in the induction of synthesis of heat-shock proteins at high temperature. We show that these cells are also defective in degrading two proteins that are normally unstable in htpR+ cells. The proteolytic defect is manifest at both 30 degrees C and 42 degrees C. We used a marker rescue technique to map this defect to the htpR locus. Although both proteolytic substrates are partially stabilized in lon- strains, we argue that the defect in proteolysis exhibited by the htpR165 strain does not mimic the lon- state. The htpR165 strain synthesizes Lon at the normal rate at 30 degrees C and does not show the phenotypes of mucoidy and radiation sensitivity associated with lon- strains. |
| Links | |
| Keywords |
Bacterial Proteins/biosynthesis; Bacterial Proteins/genetics; DNA, Bacterial; DNA, Recombinant; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Genes, Regulator; Heat-Shock Proteins/biosynthesis; Heat-Shock Proteins/genetics; Hot Temperature; Mutation; Peptide Hydrolases/genetics; Peptide Hydrolases/metabolism; Phenotype; Suppression, Genetic |
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