PMID:6386470
| Citation |
Campbell, HD, Rogers, BL and Young, IG (1984) Nucleotide sequence of the respiratory D-lactate dehydrogenase gene of Escherichia coli. Eur. J. Biochem. 144:367-73 |
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| Abstract |
The structural gene for the respiratory D-lactate dehydrogenase of Escherichia coli, a membrane-bound flavoenzyme, has been subcloned from a 7 X 10(3)-base-pair chromosomal HindIII fragment containing the gene [Young, I. G., Jaworowski, A., and Poulis, M. (1982) Biochemistry 21, 2092-2095]. The complete nucleotide sequence of the 2340-base-pair PstI-SmaI subclone has been determined on both strands by the dideoxy chain termination method. A single large open reading frame is present in the nucleotide sequence. The reading frame is preceded by a good ribosome binding site and numerous possible promoter sequences, and is followed by a typical rho-independent termination sequence. The reading frame predicts that the D-lactate dehydrogenase polypeptide consists of 571 amino acids (including the initiating methionine residue) with Mr = 64613. The protein does not have a low overall polarity, nor does it contain unusually hydrophobic stretches. It appears to contain a short repeat which is homologous with the well characterized L-lactate dehydrogenases in the vicinity of the 'essential' cysteine residue. Apart from this, homology with other proteins of known sequence has not been detected. |
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| Keywords |
Amino Acid Sequence; Base Composition; Base Sequence; Biological Evolution; Chemical Phenomena; Chemistry; Cloning, Molecular; Codon; Computers; DNA, Bacterial/isolation & purification; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; L-Lactate Dehydrogenase/genetics; Oxygen Consumption; Peptide Fragments/isolation & purification; Protein Biosynthesis; Transcription, Genetic |
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