PMID:6381645
| Citation |
Pope, NR and Cole, JA (1984) Pyruvate and ethanol as electron donors for nitrite reduction by Escherichia coli K12. J. Gen. Microbiol. 130:1279-84 |
|---|---|
| Abstract |
Pyruvate and ethanol were both effective electron donors for nitrite reduction by Escherichia coli K12. The pyruvate-dependent rate decreased by approximately 50% when either a cysG mutation, which results in loss of NADH-dependent nitrite reductase activity (EC 1.6.6.4), or a chl mutation, which results in loss of the formate-nitrite oxidoreductase activity, was introduced into the prototrophic parental strain CGSC4315. A double mutant deficient in both of these previously described activities retained only 2% of the rate of nitrite reduction of the parental strain after growth on glucose or 5% after growth on pyruvate. We conclude that any third pathway for nitrite reduction contributes little to the in vivo rate of nitrite reduction by wild-type strains. |
| Links | |
| Keywords |
Escherichia coli/enzymology; Escherichia coli/metabolism; Ethanol/metabolism; Glucose/metabolism; Nitrites/metabolism; Oxidation-Reduction; Pyruvates/metabolism |
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