PMID:6380764
| Citation |
Taylor, WE, Straus, DB, Grossman, AD, Burton, ZF, Gross, CA and Burgess, RR (1984) Transcription from a heat-inducible promoter causes heat shock regulation of the sigma subunit of E. coli RNA polymerase. Cell 38:371-81 |
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| Abstract |
The rpoD gene encoding the sigma subunit of E. coli RNA polymerase is cotranscribed with rpsU and dnaG, encoding ribosomal protein S21 and DNA primase, respectively. After temperature upshift, a heat shock promoter (Phs) located within dnaG is transiently induced, causing increased transcription of rpoD. The extent of induction is sufficient to account for the heat shock response of sigma synthesis. The initiation site of this promoter was located about 360 bp upstream of rpoD by promoter cloning and S1 nuclease mapping. Plasmid deletions generated with Bal 31 nuclease show that the DNA sequence CTGCCACCC in the -44 to -36 region of this promoter is necessary for its heat shock activity. Heat induction of transcription from Phs is under the control of HtpR, a positive regulator of the heat shock response. |
| Links | |
| Keywords |
Base Sequence; Chromosome Mapping; DNA-Directed RNA Polymerases/genetics; Escherichia coli/genetics; Gene Expression Regulation; Heat-Shock Proteins/genetics; Hot Temperature; Kinetics; Operon; Sigma Factor/genetics; Transcription Factors/genetics; Transcription, Genetic |
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