PMID:6373752
| Citation |
Rock, CO (1984) Turnover of fatty acids in the 1-position of phosphatidylethanolamine in Escherichia coli. J. Biol. Chem. 259:6188-94 |
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| Abstract |
Phosphatidylethanolamine is the major membrane phospholipid of Escherichia coli, and two experimental approaches were used to investigate the metabolic activity of the fatty acids occupying the 1-position of this phospholipid. [3H]Acetate pulse-chase experiments with logarithmically growing cells indicated that 3-5% of the acyl groups were removed from the phosphatidylethanolamine pool/generation. The reacylation aspect of the turnover cycle was demonstrated by the incorporation of fatty acids into the 1-position of pre-existing phosphatidylethanolamine when de novo phospholipid biosynthesis was inhibited using the plsB acyltransferase mutant. 2- Acylglycerophosphoethanolamine would be the intermediate in a 1-position turnover cycle, and this lysophospholipid was identified as a membrane component that could re-esterified by a membrane-bound acyltransferase. The acyltransferase either utilized acyl-acyl carrier protein directly as an acyl donor or activated fatty acids for acyl transfer in the presence of ATP and Mg2+. Acyl-acyl carrier protein was also indicated as an intermediate in the latter reacylation reaction by the complete inhibition of phosphatidylethanolamine formation from fatty acids by acyl carrier protein-specific antibodies and by the observation that the inhibition of the acyltransferase by LiCl was reversed by the addition of acyl carrier protein. Coenzyme A thioesters were not substrates for this acyltransferase. These results suggest the existence of a metabolic cycle for the utilization of 1-position acyl moieties of phosphatidylethanolamine followed by the resynthesis of this membrane phospholipid from 2- acylglycerophosphoethanolamine by an acyl carrier protein-dependent 1-position acyltransferase. |
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| Keywords |
Acetates/metabolism; Acyltransferases/metabolism; Carbon Radioisotopes/diagnostic use; Cations, Divalent; Cell Membrane/metabolism; Escherichia coli/growth & development; Escherichia coli/metabolism; Fatty Acids/metabolism; Kinetics; Membrane Lipids/metabolism; Palmitoyl Coenzyme A/metabolism; Phosphatidylethanolamines/metabolism; Tritium/diagnostic use |
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