PMID:6364142
Citation |
Ishii, S, Kuroki, K and Imamoto, F (1984) tRNAMetf2 gene in the leader region of the nusA operon in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 81:409-13 |
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Abstract |
The promoter-proximal portion of the operon containing the Escherichia coli nusA gene has been cloned. Its nucleotide sequence shows that genes for tRNAMetf2 and a 15-kilodalton protein of unknown function precede the nusA protein gene. The sequence suggests that the three genes form a single transcription unit. Consistent with this hypothesis, purified RNA polymerase formed full-length transcripts on the cloned DNA in vitro, although transcription was frequently arrested at the intercistronic site(s) between the gene for tRNAMetf2 and the 15-kilodalton protein. |
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Keywords |
Bacterial Proteins/genetics; Base Sequence; DNA, Bacterial/genetics; Escherichia coli/genetics; Gene Expression Regulation; Genes; Molecular Weight; Operon; RNA, Transfer/genetics; Transcription Factors/genetics; Transcription, Genetic |
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