PMID:6361020
| Citation |
Duchange, N, Zakin, MM, Ferrara, P, Saint-Girons, I, Park, I, Tran, SV, Py, MC and Cohen, GN (1983) Structure of the metJBLF cluster in Escherichia coli K12. Sequence of the metB structural gene and of the 5'- and 3'-flanking regions of the metBL operon. J. Biol. Chem. 258:14868-71 |
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| Abstract |
The total nucleotide sequence (1,158 nucleotides) of the metB gene of Escherichia coli coding for cystathionine gamma-synthase (386 amino acid residues, Mr = 41,503/chain) is presented. The nucleotide sequences of the flanking regions of the metB and metL genes are also presented. Analysis of these sequences and identification of a promoter region upstream from the metB gene confirms that metB and metL form an operon. The transcription direction is from metB to metL; the start site of the gene transcription has been determined. There is no structural evidence of a classical attenuation mechanism in the regulation of this operon coding for enzymes implicated in an amino acid biosynthetic pathway. Finally, the overall organization of the metJBLF gene cluster is discussed. |
| Links | |
| Keywords |
Amino Acid Sequence; Base Sequence; Cystathionine beta-Synthase/genetics; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Nucleic Acid Conformation; Operon |
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