PMID:6357954
| Citation |
Riley, M and Glansdorff, N (1983) Cloning the Escherichia coli K-12 argD gene specifying acetylornithine delta-transaminase. Gene 24:335-9 |
|---|---|
| Abstract |
The argD gene of Escherichia coli was shown to be present in plasmids pLC2-28 and pLC3-11 of the collection of Clarke and Carbon [Cell 9 (1976) 91-99]. The gene was cloned into pBR322 as a 6.3-kb BamHI fragment. Enzyme determination showed that the cloned DNA contains the structural gene for acetylornithine delta-transaminase. The argD DNA was used as a probe in hybridization experiments which indicated that the argM gene resides in a duplicated portion of E. coli DNA that is highly similar to the argD region. |
| Links | |
| Keywords |
Arginine/genetics; Cloning, Molecular; Escherichia coli/genetics; Genes, Bacterial; Operon; Plasmids; Transaminases/genetics |
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