PMID:6355770

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Citation

Hu, JC and Gross, CA (1983) Marker rescue with plasmids bearing deletions in rpoD identifies a dispensable part of E. coli sigma factor. Mol. Gen. Genet. 191:492-8

Abstract

Recombinant DNA techniques allow rapid construction and characterization of tools for genetic dissection of essential genes. In this report, we describe construction of a set of deletions in rpoD, the gene which encodes the sigma subunit of E. coli RNA polymerase. We used this deletion set to identify a region of the sigma polypeptide which is dispensable for its function.

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Keywords

Amino Acid Sequence; Chromosome Deletion; Chromosome Mapping; Escherichia coli/genetics; Genes; Genes, Bacterial; Genetic Engineering; Plasmids; Sigma Factor/genetics; Sigma Factor/metabolism; Structure-Activity Relationship; Transcription Factors/genetics; Transcription, Genetic

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