PMID:6351837
| Citation |
Lewendon, A and Coggins, JR (1983) Purification of 5-enolpyruvylshikimate 3-phosphate synthase from Escherichia coli. Biochem. J. 213:187-91 |
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| Abstract |
A procedure for the purification of 5-enolpyruvylshikimate 3-phosphate synthase from Escherichia coli is described. Homogeneous enzyme of specific activity 17.7 units/mg was obtained in 22% yield. The key purification step involves substrate elution of the enzyme from a cellulose phosphate column. The subunit Mr was estimated to be 49 000 by polyacrylamide-gel electrophoresis in the presence of sodium dodecyl sulphate. The native Mr was estimated to be 55 000 by gel filtration, indicating that the enzyme is monomeric. |
| Links | |
| Keywords |
3-Phosphoshikimate 1-Carboxyvinyltransferase; Alkyl and Aryl Transferases; Chromatography, Affinity; Chromatography, Ion Exchange; Electrophoresis, Polyacrylamide Gel; Escherichia coli/enzymology; Molecular Weight; Transferases/isolation & purification |
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