PMID:6350262
| Citation |
Vales, LD, Rabin, BA and Chase, JW (1983) Isolation and preliminary characterization of Escherichia coli mutants deficient in exonuclease VII. J. Bacteriol. 155:1116-22 |
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| Abstract |
Strains of Escherichia coli containing reduced levels of exonuclease VII activity due to mutations in the xseB gene have been isolated after mutagenesis with N-methyl-N'-nitro-N-nitrosoguanidine. Seven mutants of independent origin deficient in exonuclease VII activity were obtained. Four of these contained defects in xseA, a locus which has been previously identified, and three others contained mutations in a gene distinct from xseA, which we have designated xseB. Genetic mapping studies place the xseB locus between proC and dnaZ. Exonuclease VII purified from KLC835 (xseA+ xseB3) is more heat labile than enzyme purified from the parent strain PA610 (xse+), showing that xseB is a structural gene for exonuclease VII. The isolation of lambda transducing phage carrying xseA is also described. |
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| Keywords |
Chromosome Mapping; Chromosomes, Bacterial; Escherichia coli/enzymology; Escherichia coli/genetics; Exodeoxyribonucleases/genetics; Exodeoxyribonucleases/metabolism; Genes; Genes, Bacterial; Genotype; Mutation; Transduction, Genetic |
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