Hanahan, D (1983) Studies on transformation of Escherichia coli with plasmids. J. Mol. Biol. 166:557-80
Factors that affect the probability of genetic transformation of Escherichia coli by plasmids have been evaluated. A set of conditions is described under which about one in every 400 plasmid molecules produces a transformed cell. These conditions include cell growth in medium containing elevated levels of Mg2+, and incubation of the cells at 0 degrees C in a solution of Mn2+, Ca2+, Rb+ or K+, dimethyl sulfoxide, dithiothreitol, and hexamine cobalt (III). Transformation efficiency declines linearly with increasing plasmid size. Relaxed and supercoiled plasmids transform with similar probabilities. Non-transforming DNAs compete consistent with mass. No significant variation is observed between competing DNAs of different source, complexity, length or form. Competition with both transforming and non-transforming plasmids indicates that each cell is capable of taking up many DNA molecules, and that the establishment of a transformation event is neither helped nor hindered significantly by the presence of multiple plasmids.
Cations; DNA, Superhelical; Dimethyl Sulfoxide/pharmacology; Dithiothreitol/pharmacology; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/growth & development; Plasmids/drug effects; Species Specificity; Transformation, Bacterial/drug effects
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Add links to pages that link here (e.g. gene, product, method pages)
See Help:References for how to manage references in EcoliWiki.