PMID:6344793
| Citation |
Wovcha, MG, Steuerwald, DL and Brooks, KE (1983) Amplification of D-xylose and D-glucose isomerase activities in Escherichia coli by gene cloning. Appl. Environ. Microbiol. 45:1402-4 |
|---|---|
| Abstract |
A recombinant plasmid, designated pUC1002, was constructed by ligation of a HindIII restriction endonuclease fragment of Escherichia coli chromosomal DNA to vector plasmid pMB9. Strains carrying this plasmid were selected by transformation of an E. coli strain bearing the xyl-7 mutation to a xylose-positive (Xyl+) phenotype. Strains containing pUC1002 produced coordinately elevated levels of D-xylose isomerase and D-xylulose kinase. Under appropriate conditions, the isomerase also efficiently catalyzed the conversion of D-glucose to D-fructose. |
| Links | |
| Keywords |
Aldose-Ketose Isomerases; Carbohydrate Epimerases/biosynthesis; Carbohydrate Epimerases/genetics; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Plasmids |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.