PMID:6338477
| Citation |
Donovan, WP and Kushner, SR (1983) Amplification of ribonuclease II (rnb) activity in Escherichia coli K-12. Nucleic Acids Res. 11:265-75 |
|---|---|
| Abstract |
A 7.1 kb HindIII-XhoI fragment of E. coli DNA which contains the structural gene for ribonuclease II (rnb) has been cloned in the recombinant plasmid pDK24. At least two constitutively expressed genes are encoded on the fragment as shown by maxicell analysis. On denaturing polyacrylamide gels RNase II appears as a single 72,000 dalton species. The approximate site of transcription initiation of the rnb gene has been mapped. Although derivatives of E. coli harboring pDK24 contained 10-fold more RNase II activity that wild type strains without the plasmid, the degradation rate of mRNA was similar in all strains tested. Strains deficient in both RNase II and polynucleotide phosphorylase appear inviable. |
| Links | |
| Keywords |
Cloning, Molecular; DNA, Recombinant/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Exoribonucleases/genetics; Gene Amplification; Genes; Genes, Bacterial; Kinetics; Molecular Weight; Plasmids; Transcription, Genetic |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
See also
References
See Help:References for how to manage references in EcoliWiki.