PMID:6337148
| Citation |
Coleman, WG Jr (1983) The rfaD gene codes for ADP-L-glycero-D-mannoheptose-6-epimerase. An enzyme required for lipopolysaccharide core biosynthesis. J. Biol. Chem. 258:1985-90 |
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| Abstract |
The rfaD gene product, ADP-L-glycero-D-mannoheptose-6-epimerase, is necessary for the conversion of ADP-D-glycero-D-mannoheptose to ADP-L-glycero-D-mannoheptose. The nucleotide ADP-D-glycero-D-mannoheptose accumulates in rfaD mutant strains. Two chimeric colicin E1 plasmids carrying the coding sequence of the rfaD+ locus have been selected and shown to complement the rfaD phenotype. These clones also result in an amplification of ADP-L-glycero-D-mannoheptose-6-epimerase activity. |
| Links | |
| Keywords |
Carbohydrate Epimerases/genetics; Carbohydrate Epimerases/metabolism; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genotype; Kinetics; Lipopolysaccharides/genetics; Phenotype; Plasmids; Species Specificity; Substrate Specificity |
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