PMID:6336742
| Citation |
Arraj, JA and Marinus, MG (1983) Phenotypic reversal in dam mutants of Escherichia coli K-12 by a recombinant plasmid containing the dam+ gene. J. Bacteriol. 153:562-5 |
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| Abstract |
A recombinant plasmid, pMQ3, carrying the dam gene of Escherichia coli K-12, was constructed and transformed into dam+ and dam- strains. Both dam- and dam+ strains containing pMQ3 showed a wild phenotype for all traits, including mutation rate, except for a 10-fold increase in DNA adenine methylase activity. |
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| Keywords |
Cloning, Molecular; DNA (Cytosine-5-)-Methyltransferase/genetics; DNA, Recombinant; Escherichia coli/enzymology; Escherichia coli/genetics; Genes, Bacterial; Methyltransferases/genetics; Mutation; Phenotype; Plasmids; Site-Specific DNA-Methyltransferase (Adenine-Specific); Transformation, Bacterial |
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