PMID:6330500
| Citation |
Rosenfeld, SA, Stevis, PE and Ho, NW (1984) Cloning and characterization of the xyl genes from Escherichia coli. Mol. Gen. Genet. 194:410-5 |
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| Abstract |
Specific xylose utilization mutants of Escherichia coli were isolated that had altered xylose isomerase ( xylA ), xylulokinase ( xylB ), and regulatory ( xylR ) or transport ( xylT ) activities. We screened the Clarke and Carbon E. coli gene bank and one clone, pLC10 -15, was found to complement the xyl mutants we had characterized. Subcloning and DNA restriction mapping allowed us to locate the xylA and xylB genes on a 1.6 kbp Bg/II fragment and a 2.6 kbp HindIII-Sa/I fragment, respectively. The identification and mapping of xyl gene promoters suggest that the xylA and xylB genes are organized as an operon having a single xylose inducible promoter preceding the xylA gene. |
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| Keywords |
Aldose-Ketose Isomerases; Bacterial Proteins/genetics; Carbohydrate Epimerases/genetics; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Genes; Genes, Bacterial; Genes, Regulator; Operon; Phosphotransferases/genetics; Phosphotransferases (Alcohol Group Acceptor); Xylose/metabolism |
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