PMID:6330287
| Citation |
Britton, P, Lee, LG, Murfitt, D, Boronat, A, Jones-Mortimer, MC and Kornberg, HL (1984) Location and direction of transcription of the ptsH and ptsI genes on the Escherichia coli K12 genome. J. Gen. Microbiol. 130:861-8 |
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| Abstract |
Recombinant plasmids were constructed that carried various fragments of the DNA specifying the Escherichia coli genes ptsH and (part of) ptsI, the genes for the common components of the phosphoenolpyruvate: sugar phosphotransferase. Expression of plasmid-specified functions in minicells showed that ptsH and ptsI were transcribed clockwise. Most of the transcription of ptsI was from the ptsH promoter, but some was from a second site within or after ptsH. |
| Links | |
| Keywords |
Chromosome Mapping; DNA Transposable Elements; DNA, Bacterial; Escherichia coli/genetics; Genes, Bacterial; Phosphoenolpyruvate Sugar Phosphotransferase System/genetics; Plasmids; Transcription, Genetic |
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