PMID:6325382
| Citation |
Grossman, AD, Ullmann, A, Burgess, RR and Gross, CA (1984) Regulation of cyclic AMP synthesis in Escherichia coli K-12: effects of the rpoD800 sigma mutation, glucose, and chloramphenicol. J. Bacteriol. 158:110-4 |
|---|---|
| Abstract |
An immediate 12-fold inhibition in the rate of beta-galactosidase synthesis occurs in Escherichia coli cells containing the mutant sigma allele rpoD800 after a shift to 42 degrees C. In the present study we characterize the nature of the inhibition. The severe inhibition of beta-galactosidase synthesis was partly relieved by cyclic AMP (cAMP). We inferred that the inhibition might be mediated by a decreased intracellular concentration of cAMP. Consistent with this inference, the rate of cAMP accumulation in mutant cells after a temperature upshift was depressed relative to that in wild-type cells. Glucose and chloramphenicol, two agents known to inhibit differentially beta-galactosidase mRNA synthesis, caused a similar inhibition in the rate of cAMP accumulation. Thus, three diverse stimuli, glucose, chloramphenicol, and a temperature-sensitive sigma mutation, appear to affect beta-galactosidase synthesis by regulating the synthesis of cAMP. |
| Links | |
| Keywords |
Chloramphenicol/pharmacology; Cyclic AMP/biosynthesis; Escherichia coli/drug effects; Escherichia coli/genetics; Escherichia coli/metabolism; Genes, Bacterial; Glucose/pharmacology; Kinetics; Mutation; Sigma Factor/genetics; Sigma Factor/physiology; Temperature; Transcription Factors/physiology; beta-Galactosidase/biosynthesis |
| edit table |
Significance
You can help EcoliWiki by summarizing why this paper is useful
Useful Materials and Methods
You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.
Annotations
<annotationlinks/>
EcoliWiki Links
Add links to pages that link here (e.g. gene, product, method pages)
References
See Help:References for how to manage references in EcoliWiki.