PMID:6320721

Citation	Lawlis, VB, Dennis, MS, Chen, EY, Smith, DH and Henner, DJ (1984) Cloning and sequencing of the xylose isomerase and xylulose kinase genes of Escherichia coli. Appl. Environ. Microbiol. 47:15-21
Abstract	A 4.2-kilobase-pair fragment of the Escherichia coli chromosome which contains the genes for xylose isomerase and xylulose kinase was cloned into plasmid pBR322. The hybrid plasmid (designated pECX14) complements strains deficient in either or both of the two enzymes. Deletion derivatives of pECX14 were used to localize the two genes on the cloned fragment. The entire nucleotide sequence of the cloned fragment was determined. Open reading frames which, if translated, would encode proteins of molecular weights 54,000 and 52,000 were found. These were identified as the isomerase and kinase structural genes, respectively.
Links	PubMed PMC239604
Keywords	Aldose-Ketose Isomerases; Base Sequence; Carbohydrate Epimerases/genetics; Cloning, Molecular; Escherichia coli/enzymology; Escherichia coli/genetics; Mutation; Phosphotransferases/genetics; Phosphotransferases (Alcohol Group Acceptor); Plasmids; Protein Biosynthesis; Transcription, Genetic
edit table

Significance

You can help EcoliWiki by summarizing why this paper is useful

You can help Ecoliwiki by describing the useful materials (strains, plasmids, antibodies, etc) described in this paper.

Add links to pages that link here (e.g. gene, product, method pages)

See Help:References for how to manage references in EcoliWiki.