PMID:6319360

Citation	Daws, TD and Fuchs, JA (1984) Isolation and characterization of an Escherichia coli mutant deficient in dTMP kinase activity. J. Bacteriol. 157:440-4
Abstract	Escherichia coli LD0181 is sensitive to 15 micrograms of 2',3'-dideoxythymidine per ml. A derivative that was resistant to 40 micrograms of the same chemical per ml at 30 degrees C and that had lost the ability to grow on enriched medium at 42 degrees C was isolated after nitroso-guanidine mutagenesis. This mutant, TD105, produced a dTMP kinase with 25-fold lower specific activity and a 5-fold higher Km for dTMP than the parental strain. The dTMP pool in TD105 was 4.4-fold higher than in the parent. In addition to temperature sensitivity and resistance to 2',3'-dideoxythymidine, the mutant exhibited a hypersensitivity to 5-bromo-2'-deoxyuridine. All three of these phenotypes are cotransducible. The tmk gene was mapped by cotransduction to approximately 30 min on the E. coli map.
Links	PubMed PMC215267
Keywords	Escherichia coli/enzymology; Escherichia coli/genetics; Escherichia coli/isolation & purification; Genotype; Kinetics; Nucleoside-Phosphate Kinase/isolation & purification; Nucleoside-Phosphate Kinase/metabolism; Phosphotransferases/isolation & purification; Temperature; Thymidine Monophosphate/metabolism; Transduction, Genetic
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