PMID:6317865
| Citation |
Fayat, G, Mayaux, JF, Sacerdot, C, Fromant, M, Springer, M, Grunberg-Manago, M and Blanquet, S (1983) Escherichia coli phenylalanyl-tRNA synthetase operon region. Evidence for an attenuation mechanism. Identification of the gene for the ribosomal protein L20. J. Mol. Biol. 171:239-61 |
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| Abstract |
The nucleotide sequences of pheS and of the beginning of pheT have been determined. The genes pheS and pheT code, respectively, for the small and large subunits of phenylalanyl-tRNA synthetase, an alpha 2 beta 2 enzyme. Upstream from pheS the sequence shows another open reading frame of 354 nucleotides (rplT), which accounts for a protein of Mr 13,400. The product of this gene, previously named "P12", is identified as the ribosomal protein L20. The promoter for the pheS, T operon was located 368 nucleotides in front of pheS by transcription experiments in vitro. The promoter site is followed by a short open reading frame, which codes for a 14-residue peptide containing five phenylalanine residues. Immediately downstream from the stop codon of this open reading frame, the DNA sequence indicates that the transcript can be folded into three alternative secondary structures, one of which is a site of transcription termination. In vitro, 90% of transcription products initiated at the pheS, T promoter terminate at this site. However, long run-off transcripts proceeding through the terminator and covering the pheS structural gene are observed. No other transcription initiation could be detected between the terminator and the pheS structural gene. All these results are consistent with a mechanism by which phenylalanine-mediated attenuation controls the expression of phenylalanyl-tRNA synthetase. Further evidence is provided for this model by the features of pheS, T regulation in vivo (see the accompanying paper). |
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| Keywords |
Amino Acyl-tRNA Synthetases/genetics; Bacterial Proteins; Base Sequence; Chromosome Mapping; DNA Restriction Enzymes; Escherichia coli/genetics; Escherichia coli Proteins; Genes; Genes, Bacterial; Operon; Phenylalanine-tRNA Ligase/genetics; Plasmids; Protein Biosynthesis; Ribosomal Proteins/genetics; Transcription, Genetic |
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