PMID:6300769
| Citation |
Brooks, JE, Blumenthal, RM and Gingeras, TR (1983) The isolation and characterization of the Escherichia coli DNA adenine methylase (dam) gene. Nucleic Acids Res. 11:837-51 |
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| Abstract |
The E. coli dam (DNA adenine methylase) enzyme is known to methylate the sequence GATC. A general method for cloning sequence-specific DNA methylase genes was used to isolate the dam gene on a 1.14 kb fragment, inserted in the plasmid vector pBR322. Subsequent restriction mapping and subcloning experiments established a set of approximate boundaries of the gene. The nucleotide sequence of the dam gene was determined, and analysis of that sequence revealed a unique open reading frame which corresponded in length to that necessary to code for a protein the size of dam. Amino acid composition derived from this sequence corresponds closely to the amino acid composition of the purified dam protein. Enzymatic and DNA:DNA hybridization methods were used to investigate the possible presence of dam genes in a variety of prokaryotic organisms. |
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| Keywords |
Amino Acid Sequence; Base Sequence; Cloning, Molecular; DNA (Cytosine-5-)-Methyltransferase/genetics; DNA Restriction Enzymes; DNA, Bacterial/genetics; DNA, Bacterial/isolation & purification; Escherichia coli/enzymology; Genes; Genes, Bacterial; Methyltransferases/genetics; Nucleic Acid Hybridization; Plasmids |
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