PMID:6300094
| Citation |
Watabe, H, Iino, T, Kaneko, T, Shibata, T and Ando, T (1983) A new class of site-specific endodeoxyribonucleases. Endo.Sce I isolated from a eukaryote, Saccharomyces cerevisiae. J. Biol. Chem. 258:4663-5 |
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| Abstract |
We had found that yeasts had intracellular endodeoxyribonucleases that cut phage DNA into a set of double-stranded fragments with discrete chain lengths. We purified one of them to apparent homogeneity from Saccharomyces cerevisiae and designated it Endo.Sce I. Sequence analysis around 5 cleavage sites in plasmid DNA and phage DNA revealed that Endo.Sce I cuts a defined phosphodiester bond in each strand of double helix at the cleavage sites and produces free cohesive ends consisting of 4 nucleotides protruding at 3'-termini. However, unlike in the case of prokaryotic type II-restriction endonucleases, (i) Endo.Sce I seems to consist of two nonidentical subunits, (ii) no common palindrome or consensus sequence including more than 5 base pairs is detected at or near these cleavage sites, and (iii) Endo.Sce I can cut the DNA isolated from the cells that produced Endo.Sce I. All of the 5 cleavage sites are included in inverted repeats, but these inverted repeats are variable in size, nucleotide sequence, and distance between repeating units. An inverted repeat itself is not a structure recognized by Endo.Sce I. This study shows that Endo.Sce I is the first example of eukaryotic site-specific endonuclease and has properties, as described above, which distinguish it from prokaryotic restriction endonucleases. |
| Links | |
| Keywords |
Base Sequence; DNA Restriction Enzymes/isolation & purification; DNA Restriction Enzymes/metabolism; DNA, Viral/metabolism; Deoxyribonucleases, Type II Site-Specific; Molecular Weight; Saccharomyces cerevisiae/enzymology; Saccharomyces cerevisiae Proteins; Substrate Specificity |
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